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KMID : 0364819860240020091
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Korean Journal of Microbiology
1986 Volume.24 No. 2 p.91 ~ p.97
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Interspecific Protoplast Fusion of Trichoderma koningii and Trichoderma reesei
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Park,H.M./박희문
Jeong,J.M./Hong,S.W./Hah,Y.C./Seong,C.N./정종문/홍순우/하영칠/성치남
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Abstract
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1
Intra and interspecific fusants were produced by the protoplast fusion of aux-c otrophic mutants from Trichoderma koningii ATCC 26113 and Trichoderma reesei QM 9414. It was
found that 0.6M MgSO4 and 0.6M NH4C1 was the best osmotic stabilizer for the preparation of protoplasts from the mycelium of T. koningii and T. reesei respectively. However, MgSO4 was the most suitable one for the regeneration of the protoplasts from both species. The intraspecific protoplast fusion frequencies between the auxotrophic mutants from T. reesei were 1.8 x 10-2 to 5.1 x 10-1. Interspecific protoplast fusion frequencies between the auxotrophic mutants from T. koningii and T. reesei were 3.6 x 10-3 to 8.4 x 10-2. Interspecific complementing fusants, however, were not always produced. Fusants obtained from interspecific potoplast fusion were spontaneously segregated into various strains including parental types, non-parental auxotrophic hybrids, and prototrophic hybrids on complete plate. Interspecific hybrids revealed to have partially enhanced celluloytic activities. Key Words: protoplast fusion, Trichoderma koningii, Trichoderma reesei, interspecific hybrid
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